Detemination of blood glucose - KSU Faculty

Detemination of blood glucose - KSU Faculty

Determination of blood glucose Done by: Sahar Al-Subaie :Redox titration Redox titration (also called oxidation-reduction titration) is one type of titration based on a redox reaction between the

.analyte and titrant :Principle Cu+2 + glucose 2Cu+ +

I2 ph Cu+ + (oxi)glucose 2I- + 2Cu2 I2

I2 I2 I2 + 2S2O32- Starch 2I- + S4O62- back titration :The source of iodine It comes from acidic standard :

Potassium iodide Potassium iodate solution: KIo3 + 5KI + 6H+ 3I2 + 6K+ + 3H2o : Procedure Standardization of thiosulfate

:Analysis of sample Blank Test :Blank :Test :Titration

Titrate with thiosulfate until the color change from brown to pale yellow. Add one ml of 1% starch, the solution will turns to blue. Continue the titration untill the blue color disappear(colorless)

:CALCULATION :Standardization of thiosulfate Normality of Thiosulfate = V ( Iodate Used )X N (Iodat)

V (Thiosulphate) ml X 0.01 N ml 9 = 0.0055 N 5= :Analysis of sample(Unknown)

Meq I2 in Blank = N ThiosulfateX Volume of Thiosulphate used N X 13 ml 0.0055 = meq 0.0715 = Meq I2 in Test = N Thiosulfate X Volume of Thiosulphate used N X 9 ml 0.0055

= meq 0.0495 = Meq I2 ( reduced by Cu+) = Meq I2 in Blank - Meq I2 in Test 0.0715 = - 0.0495 meq 0.022 = Meq I2 ( reduced by Cu+) = Meq Cu2+ ( reduced by

Mg Cu2+ (reduced)= Meq Cu2+ ( reduced ) X 63.54 mg +Cu2 Meq 1 +Cu2 meq X 63.54 mg 0.022= 1meq mg 1.397

= The percent glucose in the sample is obtained directly from .(Table 1) using value for the mg Cu 2+ reduced %The percent glucose in the sample = 0.132

Recently Viewed Presentations

  • Emergency Action Plans Bureau of Workers Compensation PA

    Emergency Action Plans Bureau of Workers Compensation PA

    Flood . Security issue (hostages, robberies, etc.) Hazardous Materials incident. Fire. Building collapse. Natural gas leak. PPT-009-01. EAP considers weather events indigenous to your locale as well as emergencies resulting from breakdowns in your processes and those energy sources supporting...
  • 490dp Introduction - New York University

    490dp Introduction - New York University

    490dp Introduction Robert Grimm
  • The Victorians Meet the Modern, 1880-1917 - Quia

    The Victorians Meet the Modern, 1880-1917 - Quia

    Lacey act- allowed the president without congressioinal approval to set aside "objects of historic and scientific interest" as national monuments. John Muir- first famous voices of environmental movement, Sierra Club dedicated to preserving and enjoying mountain regions. They needed land...
  • Give them what they want: Volunteer Satisfaction & Retention ...

    Give them what they want: Volunteer Satisfaction & Retention ...

    "I want to get more teaching experience before teaching abroad." "We need a teacher every Monday! And Tuesday, Wednesday and Thursday!" "This is my neighborhood, so I really want to help my community and know it better." "José works at...
  • VLSM and CIDR - Chipps

    VLSM and CIDR - Chipps

    Author: Kenneth M. Chipps Ph.D. Created Date: 05/01/2003 09:03:04 Title: VLSM and CIDR Last modified by: Kenneth M. Chipps Ph.D. Company: Cisco Systems, Inc.
  • Circular Flow Diagram s -4% t r o

    Circular Flow Diagram s -4% t r o

    C + I + G + NX. Income. Wages, Salaries. Rent . Profits . Interest . The Circular Flow Diagram is a simplified illustration of how funds circulate through the economy. It also illustrates the relationship between three key concepts...
  • Etude Operationnelle Sur La Lutte Contre La Carence En Fer Et ...

    Etude Operationnelle Sur La Lutte Contre La Carence En Fer Et ...

    Prise en charge des problèmes de SR des élèves à l'IME et à l'infirmerie - Intégration des activités de SR de routine à travers des visites médicales systématiques : diagnostic, traitement gratuit et suivi des élèves - 4700 élèves consultés...
  • Chem. 31 - 9/15 Lecture

    Chem. 31 - 9/15 Lecture

    Chem 1A Review (very brief) First Topic: Chemical Equilibria. IntroductionInstructors. Lecture - Roy Dixon. PhD in Analytical Chemistry. Research in Environmental and Analytical Chemistry. Most of my teaching experience is in teaching Analytical Chemistry.